FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88095321
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1163890.13211711891032216TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG264950.039.377646
TATGCCG266000.038.8333749
CTCGTAT274000.038.38339645
CGTATGC274800.037.76210447
CGTATCT290700.036.95173640
TCTCGTA300200.035.02160344
TTCCGTA312700.034.84639737
TATCTCG306500.034.75094242
GTTCCGT328200.033.08987836
AGATCGG294400.032.672821
TCCGTAT334050.032.3026738
ACACGTC344700.032.29178214
CCGTATC343000.031.55194739
GATCGGA304900.031.5167312
ATCTCGT339200.031.45234143
AGTTCCG353900.031.29101835
ACGTCTG365650.030.4222716
ATCGGAA336300.028.8471013
CACACGT394700.028.47622713
CACGTCT394800.028.26466215
Produced by FastQC (version 0.11.5)