FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88095321
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1070450.1215104261893773Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA210950.045.99953550
GCCGTAT221750.043.9169748
CGCCGTA232200.041.95572347
GCGTCGT252100.041.27847312
TCGCCGT245000.039.0380746
AGCGTCG273800.038.41821711
GATCGGA274150.038.258592
CGTCGTG276650.037.50489813
CCGTATC276750.035.636649
TGGTCGC277950.034.96840343
AGAGCGT308450.034.0825049
ATCGGAA308950.034.0807233
AGATCGG306850.033.9942931
TCGTGTA313350.033.05832315
GTCGTGT322650.032.0707214
CGTGTAG326600.031.74089616
GAGCGTC334850.031.3547210
TCTCGGT339000.029.952637
GTCGCCG333200.029.87781145
GTATCAT333750.029.3797851
Produced by FastQC (version 0.11.5)