FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133744048
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA416550.045.92229550
GCCGTAT456300.042.1046648
GCGTCGT502550.038.94025812
CGCCGTA498150.038.6234747
TCGCCGT499300.038.05793446
TGGTCGC504550.037.71602243
CCGTATC529000.036.3114249
GATCGGA552800.036.0121152
CGTCGTG551350.035.76895513
AGCGTCG569900.034.44735311
AGATCGG580650.033.6704251
GTCGCCG577150.033.4149945
GTAGATC590400.033.18348332
AGAGCGT604700.032.5947769
TCGTGTA609450.032.2681315
GTATCAT602850.032.027251
ATCGGAA624350.031.8465523
GTCGTGT632150.031.18614614
GGTCGCC628400.030.70052344
GAGTGTA635500.029.87392428
Produced by FastQC (version 0.11.5)