FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219880440
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG313000.025.29304343
CGTATGC329000.024.21191844
TATGCCG385450.020.60209846
CTCGTAT396250.019.9791142
GATCGGA401150.019.8068731
TCTCGTA448900.017.8541341
ACACGTC472350.017.58327913
ATCGGAA503650.015.7167492
ATCTCGT513050.015.58078140
ACGTCTG550450.015.27331915
ACTTAGG609550.013.73972232
CACGTCT658400.012.82761114
CGTCTGA663750.012.77167616
CACTTAG668400.012.58235931
GTATGCC667700.012.28114945
ATGCCGT678950.012.18061647
CACACGT718200.011.75873912
TCGGAAG697100.011.7433743
GCACACG762200.011.54818111
TTAGGCA747450.011.45763734
Produced by FastQC (version 0.11.5)