FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219880440
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA256050.036.2424415
GCATACG278300.035.6302214
ATACGAG307200.027.813916
CGGCATA347850.026.13584712
TACGAGA356800.019.97876217
ACGAGAT399800.017.46712718
ATACGCG45450.015.99313916
GGCATAC612800.014.48230313
GCCTAAG611000.014.44278525
ACGGCAT663950.014.3505611
TGCCTAA607050.014.23513224
AGACGGC675350.014.1909249
AAGACGG680150.013.0274918
CCTAAGT487250.012.85153826
GACGGCA750900.012.525969510
CGTATCG132700.011.032493
CTCGGAT447950.010.7186431
CTCGGTT330550.010.7091471
ATGCCTA702900.010.66170923
CGCGGGT288650.010.568791
Produced by FastQC (version 0.11.5)