FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005431999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005431999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100869231
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG160350.031.2768134
TCGTATG148600.029.64278646
CGTATGC162000.026.82500847
TATGCCG162050.026.62312549
CTCGTAT169000.026.31507545
GATCGGA219250.021.7871042
AGTCACG240450.021.37031629
TCTCGTA214550.020.97357444
AATCTCG234750.020.76884742
ACACGTC250300.020.4493914
AGATCGG236550.019.9896281
ACGTTTC265050.019.61310633
TTTCGGA263350.019.54734436
GTTTCGG289250.018.60483235
GTCACGT276900.018.51903230
ATCTCGT252700.018.2238943
CGGAATC272550.018.13314239
ACGTCTG289800.017.89572116
CACGTTT296650.017.4644432
TCGGAAT287300.017.32299838
Produced by FastQC (version 0.11.5)