FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65638170
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA35653235.431783061593582TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15486942.35944116053205TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2215750.051.603821
TCGTATG5541000.047.5553644
CTCGTAT5507100.047.51541543
TATGCCG5542400.047.5087747
ACCGGCG5564250.047.4276731
CGTGAAT5433850.047.3894236
CGTATGC5562600.047.3649345
CCGGCGT5584350.047.29966432
GTATGCC5576100.047.23587446
CGGCGTG5596650.047.21841433
GCGTGAA5601350.047.13350335
TCTCGTA5433450.047.0603242
TCACCGG5602200.047.06030329
ATCTCGT5444450.046.9830741
GCCGTCT5590200.046.9753950
AATCTCG5446200.046.9640740
GTCACCG5612850.046.94581228
CACCGGC5626750.046.8715330
GTGAATC5475800.046.7733137
CCGTCTT5607700.046.7689251
Produced by FastQC (version 0.11.5)