FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65638170
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA36517645.563476251699278Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA15735102.3972484302959693Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2167800.052.570731
GCCGTAT5502300.047.68393758
CGTATCA5515050.047.61158460
CGCCGTA5501750.047.6070557
CGCGTGT5535250.047.59993437
GCGTGTA5537400.047.59453238
GGCGCGT5540200.047.5710635
TCTCGGT5546250.047.43364747
GTATCAT5542100.047.373661
GTAGATC5568750.047.3679842
TCGCCGT5525250.047.36546756
AGGCGCG5570850.047.35000234
GCGCGTG5569850.047.3252936
TGGTCGC5529800.047.29927453
CCGTATC5544000.047.29915259
GGTCGCC5530800.047.27369354
GTGGTCG5545350.047.26663652
GTCGCCG5536250.047.2470955
CGGTGGT5560750.047.19600750
TAGATCT5586900.047.19491243
Produced by FastQC (version 0.11.5)