FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140506830
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG195200.015.25635843
TATGCCG209800.014.154446
CGTATGC216400.013.87536244
CGTATCG91750.013.6132893
CTCGTAT211750.013.23478742
CCCGTAT158850.011.6684841
GTATCGA126900.011.0659134
TCTCGTA265550.010.54024741
GATCGGA267550.010.2106192
TCACGAT317350.09.92851535
ACACGTC314600.09.87575913
CGACCGT84350.09.7524381
CTCGGTT220100.09.6243971
ATCACGA353100.09.47606335
TATATCG113450.09.40094135
AGATCGG286450.09.3917921
CCGTATC172750.09.3575772
ATCTCGT313000.09.2789640
ACGATCT312100.09.25798637
CTCGGAT312450.09.0735531
Produced by FastQC (version 0.11.5)