FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140506830
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA164000.016.89686850
GCCGTAT162650.016.86728548
CGTATCG94250.014.2303593
CGCCGTA200800.014.06706147
GCGTCGT213000.013.48043812
TCGCCGT221650.012.55668446
GATCGGA232700.012.2201922
CCGTATC232600.012.13995349
GTATCGA120750.011.8328484
CCCGTAT160050.011.7506921
CGACCGT94000.011.4008811
CGTCGTG254850.011.32216213
AGCGTCG257700.011.12831311
TGGTCGC262550.010.65487143
TCGTGTA310050.09.93815815
ATCGGAA284750.09.924493
GTCGTGT323300.09.67146314
CGATAAC147600.09.6572371
GTCGCCG300700.09.60533745
TATATCG113300.09.57999735
Produced by FastQC (version 0.11.5)