FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106387223
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG241350.033.96621346
CTCGTAT255450.031.75963645
CGTATGC271250.030.45363647
TATGCCG274850.030.02943449
GTCACGA292250.028.67763730
TCTCGTA292500.027.66450744
CACGAGT308000.027.21146232
AGTCACG319450.026.23582629
TATCTCG316300.025.61637342
AGATCGG302350.025.4907261
ACACGTC330400.025.47752814
ATCTCGT324050.024.94938743
GATCGGA311750.024.7717292
ACGAGTG349550.023.78510933
TCACGAG355650.023.76394731
ACGTCTG363450.023.25526616
CACGTCT387950.021.7686715
ATCGGAA355650.021.706583
CACACGT425250.020.16361213
CGAGTGG423900.019.90597234
Produced by FastQC (version 0.11.5)