FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106387223
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA190750.040.77647450
GCCGTAT197700.039.34881648
GCGTCGT219000.036.28393612
CGCCGTA243350.032.0324347
TGGTCGC241900.031.84008643
GATCGGA260750.031.3035872
TCGCCGT263250.029.20352246
CCGTATC277650.028.46927549
AGAGCGT283300.028.1792689
GTAGATC296200.027.27155332
GTCGCCG289300.027.16785245
CGTCGTG304150.026.60976613
AGCGTCG302150.026.41409111
ATCGGAA308550.026.3736933
AGATCGG310250.025.6674291
TCTCGGT314150.025.64655737
GTATCAT338050.023.47405251
GAGCGTC351550.022.73375310
TCGGTGG354050.022.60872739
AAGAGCG364700.022.227158
Produced by FastQC (version 0.11.5)