FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107062174
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA109750.013.67683450
CGTATCG78950.013.0366593
GCCGTAT122450.011.71745748
CGCCGTA124700.011.56537347
GCGCTAA106850.011.40434342
TATATCG89950.011.32846335
GTATCGA93550.011.3018434
ATATCGC99100.010.67176936
CGCTAAG119050.010.58777543
CGATAAC100950.010.3006811
CCCGTAT125650.09.7804861
CGACCGT61400.09.6938511
CGGAAAT208300.09.5303661
CGCGGAT76400.09.3487291
GTCGTTA95000.09.21198520
CCGGGAT223650.09.20500851
CGCAAAT115650.09.2033041
CGCGGTT87500.09.0030621
TCGCCGT162400.08.96832946
CGCGAAT37950.08.9490251
Produced by FastQC (version 0.11.5)