FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149205716
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG208650.030.93445640
TCGTATG308350.030.04826743
CGTATGC323700.028.52743744
CTCGTAT337450.027.37555342
TATGCCG349050.026.4751446
AACGGTA252950.025.58323943
TATCTCG360950.024.9655139
GTCGAAC259500.024.9540639
CGAACGG262500.024.59999841
ACGGTAA272200.023.8373444
TTGAACG297850.023.4156077
GATCGGA408650.022.9527951
CGGTAAC292850.022.1682145
CGCCAAT428500.021.86204733
GAACGGT303850.021.43406342
AGTCACG438700.021.28747628
AGTCGAA309600.021.05000138
ACACGTC464250.020.49030113
GAACGCT324800.020.4523649
GTCACGC462900.020.17026329
Produced by FastQC (version 0.11.5)