FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149205716
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA316650.028.91462349
TCGAACG202550.028.61342840
GCCGTAT328700.027.75047947
CGCCGTA388000.023.71552746
AACGGTA246700.023.3112243
CGAACGG256550.022.65787541
GTCGAAC256350.022.6082239
ACGGTAA262350.022.19753544
TCGCCGT422350.021.93237345
CCGTATC420850.021.7722348
GATCGGA425350.021.7129291
TTGAACG291050.021.712057
GCGTCGT434050.021.3383111
CGGTAAC283100.020.65587845
GAACGGT295350.019.81000142
TGGTCGC469100.019.7678542
GTCGCCG477800.019.62531944
GAACGCT316350.019.1360329
AGTCGAA310550.018.89528838
TCGTGTA493850.018.7407214
Produced by FastQC (version 0.11.5)