FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123822857
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2259080.1824445061867697TruSeq Adapter, Index 9 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1329420.10736466854419292TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG710250.033.7883743
CTCGTAT696500.033.7475742
TATGCCG709650.033.58825746
CGTATGC737300.032.5487344
GATCGGA759550.031.9013841
GTCACGA765100.031.2072829
ACACGTC777250.030.925413
TTGAACG357600.030.5088637
ACGATCA789550.030.33725732
AGTCACG791550.030.18596828
TCGAACG350300.030.01634240
ATCTCGT770550.029.41355540
ACGTCTG823600.029.27266115
CACGATC826500.028.95955831
CGATCAG835350.028.7069133
TCACGAT833650.028.6908130
ATGCCGT836000.028.40968347
CACGTCT861750.027.98127214
ACGGTAA377500.027.91582944
CGTCTGA878300.027.45735416
Produced by FastQC (version 0.11.5)