FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123822857
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA587350.038.7125149
GCCGTAT614950.036.89750347
CCGTATC648150.035.0071748
CGCCGTA676800.033.6248746
TCGCCGT675850.033.45549845
GATCGGA726950.031.5987631
TCGTGTA712550.031.48650414
TGGTCGC725150.031.46981842
GTCGCCG731600.031.13712144
GTCGTGT732950.030.61562313
GCGTCGT743100.030.47506311
TTGAACG332500.030.324137
GTATCAT758150.030.20546750
GGTCGCC765500.029.83373843
AGAGCGT771100.029.6322658
TCGAACG325100.029.46785540
CGTCGTG778150.029.17809712
CGTGTAG790750.029.02228515
GAGTGTA827300.028.66073627
GTAGATC820200.028.56714831
Produced by FastQC (version 0.11.5)