FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48133478
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA521550.1083549374927779No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG518410.10770258488281274TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84850.027.84480544
CTCGTAT87800.026.93390843
TCGAACG93450.025.68089140
TTGAACG100750.024.8878827
AGATCGG97750.024.3202721
GAACGCT104200.023.5774789
ACGGTAA104000.023.37333544
ACCGATG104150.022.81070133
TATCTCG100600.022.78423340
GATCGGA104400.022.7405592
GTCGAAC106700.022.4301839
AACGGTA111650.021.88974843
CGATGTA108600.021.75574135
ATCTCGT106400.021.64560541
GAACGGT111900.021.52596742
AACGCTG116750.021.2690910
TCGACTA36750.021.19032944
ACACGTC115400.020.83205414
AGTCGAA116150.020.7762238
ACGTCTG118100.020.37623816
Produced by FastQC (version 0.11.5)