FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51502609
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG46450.019.8909023
TCGTATG73550.019.3684443
CGTATGC79600.017.50697744
TATGCCG79000.017.45940446
CTCGTAT78050.017.36741342
GTATCGA56350.017.0683234
CCCGTAT72750.014.7877631
CCGTATC71700.014.6170542
TCTCGTA92750.014.50355941
TATATCG45900.013.82669835
GATCGGA100600.013.7101731
ATCTCGT103350.013.2159440
ACACGTC111950.012.96379613
ATATCGC51750.012.59743536
ACGATCT112800.012.16996937
TCACGAT121100.012.01936935
ACGTCTG119600.011.87515815
CAATCGG60850.011.8403954
GCGCTAA42250.011.592542
CGCGAAT18200.011.5568471
Produced by FastQC (version 0.11.5)