FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51502609
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG45300.017.5048643
CGTATCA60250.015.46205249
GTATCGA56700.014.8979374
GCCGTAT62900.014.70023347
CCCGTAT70700.013.1659391
GCGTCGT76850.012.34314711
TATATCG45700.012.23295335
CGACCGT26300.012.1908711
GCGCTAA41800.011.55657542
ATATCGC52250.011.29385836
CCGGGAT103600.011.2809961
AGCGTCG91200.011.11864710
CGATAAC55650.011.0890761
CGCCGTA83450.011.07869946
CGCGGTT28100.010.7965221
GTCGTTA47450.010.76294920
ATCGCGC74250.010.73387267
GATCGGA96100.010.7264331
CAATCGG63500.010.6963494
CGCTAAG47150.010.61122143
Produced by FastQC (version 0.11.5)