FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78022535
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG98600.017.95102743
TATGCCG101600.017.21741146
CGTATGC108450.016.12973644
CTCGTAT107600.015.9364642
CGTATCG61750.012.6937933
TATCTCG143300.012.44631839
ACCGATG158300.012.31253932
GATCGGA147300.012.2681
TCTCGTA144950.012.18612241
GTATCGA71300.011.7684514
ACACGTC167400.011.66310913
ATCTCGT160600.011.12712540
ACGTCTG177950.011.068583515
CGACCGT39750.011.0392961
CCCGTAT93450.011.0182291
CGATGTA194050.010.20411434
TCACCGA201350.09.9030230
CACCGAT195600.09.85901131
GCGCTAA64200.09.78101942
CGCGGGT101450.09.63849451