FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78022535
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT76050.017.28269448
CGTATCA75700.016.95341750
CGTATCG58200.014.9403263
GCGTCGT103100.013.39224812
GTATCGA72850.012.6467044
GATCGGA119000.011.8291552
AGCGTCG119300.011.66033211
TCGCCGT116000.011.47895746
CCCGTAT96050.011.3141511
CGACCGT41300.011.1099051
CGCCGTA125700.010.64826247
CCGTATC131150.010.44299749
CTCGGAT167250.010.2724021
CGTCGTG140100.010.12502913
CAATCGG96300.010.1045854
AATCGGG108150.010.082245
TGGTCGC133850.09.97335343
TCGATTG82500.09.87001420
AGAGCGT144450.09.7946459
CGACGAT56100.09.7778421
Produced by FastQC (version 0.11.5)