FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53300251
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6566921.2320617401970584TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6422431.2049530498458627TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA941850.052.5292131
TATGCCG1494750.034.2882847
CGTATGC1518100.033.75783545
TCGTATG1518450.033.73487544
CTCGTAT1524600.033.51257743
ACCGGAA1550200.032.9944231
GTATGCC1566200.032.76459546
CACCGGA1564800.032.67741830
CGGAACT1566000.032.5405633
TCACCGG1580350.032.44721629
CCGGAAC1584400.032.2516732
GTCACCG1600850.032.0108728
ATGCCGT1596000.032.00846548
CGTCTGA1599950.031.98424115
ACGTCTG1605900.031.88077414
GCCGTCT1599350.031.83512550
TCTCGTA1488150.031.82703842
ACACGTC1620150.031.66430512
TGCCGTC1641550.031.12708749
ATCTCGT1520500.031.1248441
Produced by FastQC (version 0.11.5)