FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67850527
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5489860.8091108857562743TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2125290.31323117062893263TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG844200.047.52918644
TATGCCG845850.047.4375947
CGTATGC857000.046.85979545
CTCGTAT868250.045.9227143
AACGTTC890750.045.02472733
GTATGCC902150.044.56038346
CACAACG907350.044.21000330
CAACGTT917050.043.77860632
TCTCGTA911100.042.8352642
ACGTTCC938750.042.7668234
CGTCTGA941050.042.69646515
ACACGTC949000.042.45151512
ACAACGT945350.042.44706731
ATGCCGT948050.042.38249648
ACGTCTG952400.042.2202414
ATCTCGT928500.041.99908441
GCCGTCT957450.041.75380750
CGTTCCA973000.041.2357135
CCGTCTT983550.040.64931551
CACGTCT1006100.040.0489413
Produced by FastQC (version 0.11.5)