FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73908577
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14205331.9220137332640026TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5899220.7981779976632483TruSeq Adapter, Index 2 (97% over 36bp)
GGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGC3500640.4736446217872657No Hit
GGGGTTTAGTGGTACTTGTGAGCCAGGGCAGCGGCCACTCCAGCCACCACCTTCTGGAAGGCAGCCTGTGCAGCG3291950.445408386092997No Hit
GTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCC2814610.3808231891678824No Hit
CCCAGGGCCTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCA2704750.36595887917041076No Hit
GCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCA2701820.3655624434495607No Hit
GGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGTG2678330.36238419256806964No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAG2619940.35448389163276683No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCAGCGGCCACTCCAGCCACCACCTTCTGGAAGGCAGCCTGTGCAGCGGGG2205160.29836320620812384No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTACA2095940.28358548967868774No Hit
CTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCAGTCAGGTG2087130.28239347647026136No Hit
GGGGGTTTAGTGGTACTTGTGAGCCAGGGCAGCGGCCACTCCAGCCACCACCTTCTGGAAGGCAGCCTGTGCAGC2085730.28220405325893366No Hit
GGCAAGAGCAGGAAAGGGGGTTTAGTGGTACTTGTGAGCCAGGGCAGCGGCCACTCCAGCCACCACCTTCTGGAA2033160.27509121167357886No Hit
GTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGC1951950.26410331239363466No Hit
CCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACA1904600.2576967487819445No Hit
CAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGCAAGAGCAGGAAAGGGGGTTTAGTGG1817170.24586726923453012No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTACATATTCAGCACCATGG1767150.23909944849837927No Hit
GTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGG1692080.22894230530240084No Hit
CCAGGGCCTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCAG1636940.22148173682196587No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCA1607070.21744025730599578No Hit
GGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCA1601690.2167123309653222No Hit
GGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAAT1600060.21649178822641924No Hit
GAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGC1588430.21491822254946133No Hit
GGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGGCAGTTATCACTTTCTTGCCATGGGC1585130.21447172497990322No Hit
GGCCTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCAGTCAG1576080.21324723922096347No Hit
CAGGGCCTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCAGT1394900.18873316962928405No Hit
CTTCACTTTGGCATTACCCATGATAGCAGAGGCAGAGGATAGGTCTCCAAAGCTATCAAAGTACCGCTGGGTCCA1374780.18601088747791747No Hit
GTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCT1359670.18396646981851647No Hit
CTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGC1318480.17839336833666816No Hit
GTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCC1307670.17693075054063076No Hit
GGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAG1216840.1646412431942777No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGG1173010.1587109436567829No Hit
GCCCAGGGCCTCACCACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATC1165250.15766099785685225No Hit
CCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTG1156380.15646086651079752No Hit
GCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAG1132610.15324473098704092No Hit
GCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTC1121170.15169687274590607No Hit
GCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCG1083860.14664874416402307No Hit
GTGCAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGT1080360.14617518613570385No Hit
CTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTC1073030.14518342032210957No Hit
GGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCAT1025080.13869567533413613No Hit
CACCAACTTCATCGGCGTTCACCTTTCCCCACAGGCCAGAGACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCA1001770.13554177886553004No Hit
GGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT976060.1320631568917908No Hit
CCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCA959210.1297833132411682No Hit
GAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTC954810.12918798314842403No Hit
CAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGT936560.12671871628647377No Hit
GCCTGTGCAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTG935050.12651440982282747No Hit
TGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGGCAGTTATCACTTTCTTGCCATGGG901370.12195742856745842No Hit
AAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAG892580.12076812140490811No Hit
CTCCAAAGCTATCAAAGTACCGCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCACCACCAACTT879780.11903625204419778No Hit
CTTTATCATTTTGCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGCAAGAGCAGGAA873080.11812972667570099No Hit
CGGCGATCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGG858130.11610695738330885No Hit
GAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTACATATTCAGCACCAT839310.11356056821388945No Hit
CCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCA819120.11082881490195652No Hit
CCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGCAAGAGCAGGAAAGGGGGTTTAGTG812040.10987087466181361No Hit
CCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCT807760.1092917808443261No Hit
GTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTACATATTCAGCACCATGGCCACCAATC805260.10895352510981236No Hit
CAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAG801570.10845425964567008No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTTATCATTTTGCCAACAACTGACAGAT788620.10670209494088893No Hit
CTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACAT788020.10662091356460562No Hit
GCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGGCAGTTATCACTT786070.10635707409168492No Hit
GTCTTTATCATTTTGCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGCAAGAGCAGG781760.10577392120538325No Hit
CGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAA780370.10558585101699361No Hit
GGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTA776550.10506899625465661No Hit
GGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGT774490.1047902735294173No Hit
GGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAG761890.10308546462746805No Hit
GCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCT759370.10274450284707823No Hit
GCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGCAAGAGCAGGAAAGGGGGTTTAGT758180.10258349311744969No Hit
GGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGG750230.10150783988169601No Hit
GCTTGTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAA749400.10139553897783744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2133400.049.4292141
TCTCGTA2236700.045.77855743
TATGCCG2291550.045.5873148
TCGTATG2299850.045.37470645
ATCTCGT2268450.045.1377642
CGTATGC2315900.045.0832946
CTCGTAT2314150.045.00658844
GTATGCC2331750.044.78111647
GCCGTCT2339300.044.6254651
TAACATC2310700.044.34722538
CATCTCG2324300.044.0826741
CCGTCTT2375800.043.928152
TTAACAT2358100.043.49411437
ACATCTC2370000.043.23668340
CGTCTTC2425300.043.03278753
ATTAACA2428400.042.9824936
ATGCCGT2435850.042.91806449
ACCAATT2432650.042.90840532
TGCCGTC2444250.042.79009250
AACATCT2398000.042.76754439
Produced by FastQC (version 0.11.5)