FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73908577
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA9578361.2959740788947947Illumina Single End PCR Primer 1 (97% over 43bp)
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTG5947900.8047645133256996No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCG4974750.6730950861088829No Hit
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGG4551840.6158743930356013No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCC2870270.38835411484109617No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCAT2636400.35671096738880526No Hit
CGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGC2107340.2851279358280704No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGA1892660.25608123939390687No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTG1867360.2526580913606279No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGT1731740.23430839427472674No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGG1646700.2228022872095075No Hit
CTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAA1593890.21565697307363935No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGT1411250.19094536213300387No Hit
CTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCCTTGCCTCTGTGAG1402610.1897763503145244No Hit
CCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGC1300740.1759931056445587No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAG1238750.16760571645155609No Hit
GTTGTGTTGACTTGCAACCTCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGG1218060.16480631199272042No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGG1177150.15927109515313764No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC1172960.15870417854209262No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGT1168420.1580899061282157No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCT1143780.1547560576088483No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCT1118250.15130179004799402No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTTCACCCCCGCGGTGCA1111250.15035467399135558No Hit
GGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACAGGCTGCCTTCCA1095730.14825478239149428No Hit
GCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAG1080460.1461887163650844No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCT1070320.14481675110589667No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGAAAGGTGAACGCCGATG1053490.14253961350115021No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTC1034160.13992422016189002No Hit
GTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGC1022970.13841018749420653No Hit
CTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACA1013210.13708963710666489No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC1002310.135614842104185No Hit
CCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAA963590.13037593728803626No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTG942660.12754406027868728No Hit
CGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACAGGCTGCCTTCCAGAAGGTGGTGGC941110.12733434172328878No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAG924820.12513026735719726No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCT912420.12345251891400914No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCAG891690.12064770236342123No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTG879000.11893071625502952No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGAAA878200.11882247441998511No Hit
ATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACC876440.11858434238288744No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGGA872460.11804583925354158No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA869480.1176426384180012Illumina Single End PCR Primer 1 (97% over 42bp)
GTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCT864930.1170270129811862No Hit
CCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCT864320.11694447858196484No Hit
TGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCACCCTGCCGATTT820440.11100741392977977No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGTAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCA807660.10927825061494555No Hit
CCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCA798660.1080605299706961No Hit
GCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAA785810.1063218954952955No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTAGCCACCA782160.10582804212290545No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAAC762730.1031991185542647No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGG745720.10089762653663321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1342750.056.58226858
TCGCCGT1360550.054.98657657
CCGTATC1448400.054.11607460
GCCGTAT1480700.053.8499859
CGTATCA1497850.053.6750961
TGGTCGC1480000.053.03223454
GTATCAT1525050.052.8432862
GTAGATC1878650.051.62084643
GATCTCG1825550.051.5634646
GTGGTCG1568950.051.53639653
TAGATCT1897100.051.08819244
AGATCTC1859600.050.76164645
GATCGGA2066050.050.6596531
CGGTGTA2002400.050.20291539
ATCATTA1717400.049.93896564
CGGTGGT1685650.049.93872551
GTGTAGA1967300.049.84790441
TATCATT1619400.049.80647763
TGTAGAT1972200.049.68232742
TCATTAA1745100.049.6322165
Produced by FastQC (version 0.11.5)