FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74351914
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84300.015.07034145
CGTATGC93800.013.76788746
TATGCCG93100.013.45960548
CTCGTAT98750.012.90044944
GTATCGA66950.012.2371574
CGTATCG56250.011.7639943
TATATCG68550.011.38538135
CGATAAC64400.011.2601551
ATATCGC74200.010.895514536
TATCTCG124800.010.68523941
CCCGTAT82050.010.6311611
CGCGGAT52800.010.5492871
CGACCGT38500.010.19100951
CGCGAAT25000.010.0890991
TCTCGTA136400.09.77674743
CCGGGAT150850.09.5910161
CGGAAAT144350.09.4889871
CTCGGAT164450.09.41560651
CGGGAAT119550.09.347621
CTCGTTT123900.09.2173531
Produced by FastQC (version 0.11.5)