FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74351914
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG56400.014.3485433
CCCGTAT85250.013.358111
GTATCGA64100.013.0463994
TATATCG70950.012.75169535
ATATCGC77000.011.74976736
CGACCGT39000.011.4218711
GCCGTAT74400.010.75113148
CGATAAC70100.010.7104671
CGCCGTA80750.010.16521647
CGTATCA79450.010.11174550
TATCGCT92800.09.71386937
CTCGGAT160800.09.4053771
CTCGGTT112500.09.2603131
GACGCTA62900.09.2001224
CGCGGAT56850.09.1625791
CTCGGGT144850.09.0026071
GTCGTTA70600.08.859755520
ACGCTAC67800.08.8487255
CTCGTTT130450.08.7571581
GCGCTAA58250.08.69531442
Produced by FastQC (version 0.11.5)