FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104287135
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT265850.043.6486144
TCGTATC327800.034.8608838
GATCGGA352300.034.1678851
ATTCGTA340650.033.69705236
TATCTCG342550.033.56744841
GTCACGA358150.032.67540729
CGTATCT353600.032.41366639
TCTCGTA359650.032.20153843
ATCGGAA378900.031.7851662
GAATTCG365150.031.74898534
CACGAAT370900.031.3637231
TTCGTAT368850.031.36108837
AATTCGT368500.031.23564735
CGTCTGA380000.031.22280116
ACGAATT373100.031.15619532
CGAATTC373050.031.04585533
ACACGTC385850.030.71300513
ATCTCGT386750.030.01907542
AGTCACG393100.029.97352828
ACGTCTG397450.029.85196715
Produced by FastQC (version 0.11.5)