FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104194717
Sequences flagged as poor quality0
Sequence length50-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT120800.034.4113812
CGTCGTG127600.032.62298213
AGCGTCG137750.030.73605211
TCGTGTA138050.028.45711315
AGATCGG155600.027.65411
GTCGTGT149250.026.79865614
CGTGTAG164300.024.17171716
GATCGGA188550.022.9304642
AGAGCGT193200.022.126319
GAGCGTC200050.021.25115610
TCTTCGC172050.021.17273333
TTCGCCT176600.020.88618535
ATCGGAA212400.020.4383963
GTCTTCG183450.020.2302732
AAGAGCG227100.018.951378
TCGCCTG201850.018.45712736
CTTCGCC208500.018.36270734
TCGGAAG277800.015.75013354
CGCCTGT254700.014.76139937
CGGAAGA304300.014.5857215
Produced by FastQC (version 0.11.5)