FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159223092
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT857050.057.32166344
AGATCGG526200.046.6582341
TCTCGTA1271950.038.4779343
GATCGGA1264900.037.215281
ATCTCGT1301850.037.12837642
GTCACGA1259900.036.26804429
ATCGGAA1320000.035.7010082
CGAGATT1287250.035.28080433
ACACGTC1316150.035.14538213
CGTCTGA1306750.035.03914316
ACGAGAT1292300.034.95688632
AGTCACG1312800.034.92099428
CATCTCG1389850.034.91571441
TCACGAG1324200.034.53045730
ACGTCTG1339300.034.2635215
CACGAGA1323650.034.236131
TCGGAAG1425900.033.0605433
CACGTCT1394600.033.03748314
GCACACG1421850.032.7642811
CGGAAGA1470100.032.05974
Produced by FastQC (version 0.11.5)