FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159223092
Sequences flagged as poor quality0
Sequence length50-75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG535000.045.8458021
GCGTCGT542650.044.3891712
CGTCGTG561950.042.8913813
TCGTGTA570250.041.9480615
AGCGTCG588550.041.1499811
GTCGTGT598450.040.0533114
CGTGTAG645650.037.18877816
TAGGCTA644100.035.51315733
AGAGCGT689200.035.3313269
GATCGGA698450.035.3128242
GAGCGTC704500.034.5901210
ATCGGAA740150.033.333073
GCTATAG685550.033.33284836
GGCTATA742550.031.5515735
AAGAGCG784850.031.186748
CTATAGT699450.030.30837437
GAGTGTA733650.029.83227328
GTAGGCT779500.029.70251832
TAGTGTA647750.027.99583640
TCGGAAG887400.027.8879554
Produced by FastQC (version 0.11.5)