FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125279619
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161000.010.84132547
TCGTATG172400.010.55532844
CTCGAAT243950.010.4890461
CTCGTAT177650.010.18408443
CGGAAAT212450.010.1644231
CATACGA120500.09.54083757
CGTATGC189700.09.51873145
CCGGGAT278050.09.1009851
TACGATA122900.09.09940759
ATACGAT124550.09.09073958
CCGGAAT221900.08.9192631
CGACAAT124850.08.8604141
GCATACG133300.08.80759456
CGACGAT156450.08.787641
CGCGGAT86500.08.7437131
CGGGGAT258400.08.73990251
CGAATGT210900.08.7085013
CTGGAAT921650.08.6549351
AATCTCG214150.08.6451940
CTCGGAT374250.08.480331
Produced by FastQC (version 0.11.5)