FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125279619
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA119300.016.01312350
CGCCGTA132300.014.23327347
GCCGTAT141900.013.68622548
CTCGAAT251500.011.7556661
CCGTATC169200.011.64262549
TCGCCGT187350.010.33298946
TCGTGTA199350.010.15887315
ATACGAT122500.010.11087558
TACGATA121150.09.93473859
CATACGA130350.09.71683157
CGCGAAT55400.09.674411
GCGTCGT202150.09.55053212
GTCGTGT213850.09.4700414
CGACGAT163250.09.205491
CGAATGT208800.09.1091333
CGATACT135900.09.08793461
CGTGTAG235400.09.06372216
CTCGGTT249000.08.9896811
CGTCGTG220650.08.98795213
TCGAATG210300.08.92804052
Produced by FastQC (version 0.11.5)