FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138985591
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG280400.022.69553644
CTCGTAT287600.021.77339643
TATGCCG308550.020.88881347
CGTATGC310550.020.67315545
AGATCGG311800.019.5296061
GTCACGA355500.018.63812430
TCTCGTA350050.017.65838642
GATCGGA353900.017.301992
AGTCACG395650.016.70220429
ACACGTC398050.016.61038614
ACGATCA400750.016.5602133
TCACGAT409300.016.20553631
CGATCAG430800.015.51138634
ATCTCGT408100.015.31864541
ACGTCTG440950.015.05168816
ATCGGAA410450.014.9389453
CACGATC453050.014.8740732
ATGCCGT465100.014.12024148
CACACGT484200.013.90699713
CACGTCT480350.013.87517615
Produced by FastQC (version 0.11.5)