FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138985591
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT206300.029.63489748
CGTATCA214200.028.68389750
GCGTCGT242750.025.9896112
TCGCCGT266400.022.93352346
CGCCGTA268700.022.79821447
AGCGTCG286550.022.26063311
GATCGGA291850.022.0364782
CCGTATC285800.021.69527249
CGTCGTG295100.021.67626813
TGGTCGC301400.020.51570543
AGATCGG312450.019.9348891
GTCGCCG323800.019.35525745
AGAGCGT338000.019.1114229
ATCGGAA349550.018.46823
GTAGATC374900.017.44857832
TCGTGTA377150.016.97913215
GTCGTGT385300.016.73795914
CGTGTAG401900.016.1771616
GTATCAT408800.015.46568451
GAGCGTC416350.015.41414710
Produced by FastQC (version 0.11.5)