FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69028972
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13246841.9190261155852069TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3215830.46586670883639986TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1779450.053.8395147
TCGTATG1804900.053.1783644
TACCTTA1812350.052.87938735
TATCTCG1798750.052.55899440
CGTATGC1829850.052.4705845
ACGGTAC1840700.052.1507931
CTCGTAT1838800.052.1453243
CGGTACC1847700.051.9513532
AGTCACG1866650.051.3479927
ATGCCGT1878200.050.97393448
GTATGCC1886500.050.8480146
TCTCGTA1860600.050.8334242
ACCTTAT1863350.050.7815836
GCCGTCT1902150.050.28859350
CACGGTA1911050.050.2815530
ACACGTC1915100.050.17918412
ACGTCTG1916300.050.13263314
CGTCTGA1920300.049.99568615
TGCCGTC1916900.049.9754249
GTCACGG1926550.049.82848428
Produced by FastQC (version 0.11.5)