FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69028972
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA13494801.9549472647513857Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA3285050.4758943824340887Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1778400.053.6669758
CGTATCA1794850.053.16963660
CCGTATC1796450.053.1318859
TCGCCGT1810200.052.6965256
CGCCGTA1815500.052.62637357
GTCGCCG1842050.052.01199755
ACGTCTT1847600.051.98971633
TCTCGGT1848200.051.9573347
GTGACGT1856850.051.76640730
GTAGATC1857550.051.7487242
CGTGTAG1860650.051.72254614
CGTCTTG1858650.051.69566334
GATCTCG1859300.051.6720745
GCGTCGT1865350.051.63935510
TGTGACG1860000.051.6233929
ATCTCGG1866850.051.4819146
GTATCAT1857050.051.4261661
GACGTCT1874500.051.24404532
GTAGGGA1883950.051.11948417
CGTCGTG1886100.051.0675911
Produced by FastQC (version 0.11.5)