FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65739634
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA2861080.4352138620059856TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1991640.30295879043074686TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT687400.036.0184244-45
TATGCCG698500.035.59674548-49
CGTATGC736150.033.8605546-47
ATCTCGT736400.033.6280342-43
AGATCGG920700.031.5158481
CCGTCTT838200.029.75054652-53
TGCCGTC856200.029.12517250-51
ATCGGAA1035850.028.0789763
GATCGGA1039450.027.992612
CGTCTGA922250.027.5667516-17
CACGTCT925550.027.47857314-15
CACACGT927400.027.43652212-13
ACATGTC928200.027.34651432-33
TCGGAAG1119950.026.1254354
CGGAAGA1145000.025.7589055
GAATCTC976950.025.61970740-41
TCACATG1022650.024.91042130-31
ATGTCAG1074000.023.74039534-35
GTCAGAA1092950.023.287836-37
AGAGCAC1309800.022.7847429
Produced by FastQC (version 0.11.5)