FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250560064
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG780550.025.29804445
TATGCCG797550.024.6128948
CGTATGC825150.023.97966646
GATCGGA779600.023.9582791
CTCGTAT805800.023.86501544
TCTCGTA913050.021.1001843
AATCTCG932000.020.9574541
ATCGGAA900000.020.717362
ACACGTC1029600.020.54336513
ACGTCTG1063200.019.78541815
ATCTCGT1007700.019.1810842
CACGTCT1164700.018.1367614
CACACGT1187850.017.83282912
AGATCGG601000.017.3424451
TCGGAAG1098300.017.1666873
ATGCCGT1148400.017.10246749
GTATGCC1215050.016.64663347
GCACACG1333000.016.28894811
GCCGTCT1241950.015.89850351
CGTCTGA1351750.015.75808416
Produced by FastQC (version 0.11.5)