FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250560064
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT369550.028.73107548
CGTATCA398550.026.82007250
CGCCGTA453050.023.5272247
GCGTCGT482750.022.67401712
CCGTATC499300.021.66896849
TCGCCGT488750.021.56910746
GATCGGA542200.021.3650262
CGTCGTG539000.020.6004813
TGGTCGC532450.019.92506443
AGCGTCG597250.018.41458911
AGAGCGT624800.017.7413339
AGATCGG617600.017.6755121
GTCGCCG627450.017.40987245
ATCGGAA668150.017.3998763
GTCGTGT707050.016.72808314
TCGTGTA721600.016.36658315
CGTGTAG732200.016.26814816
GTAGATC700650.015.90407532
GAGCGTC737600.015.01580810
TCTCGGT758600.014.67051137
Produced by FastQC (version 0.11.5)