FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152273046
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2481850.16298682302579012TruSeq Adapter, Index 9 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1525810.10020223802444984TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG774000.033.0331543
CTCGTAT781050.032.23918542
CGTATGC797400.032.0628544
TCGAACG390950.031.38670240
TATGCCG812700.031.36731346
TTGAACG421550.030.3427817
GTCACGA854300.030.045729
GATCGGA886950.029.2442191
ACACGTC902450.028.72583813
ACGATCA908050.028.29879232
AGTCACG920050.027.98029928
ATCTCGT862650.027.93209640
CGGTAAC441750.027.92421345
ACGGTAA444200.027.79482744
GTCGAAC448900.027.41879339
ACGTCTG951700.027.29061715
TCACGAT948800.027.11544430
CACGATC954000.026.92862131
CGAACGG464100.026.46934341
CGATCAG986850.026.15061633
Produced by FastQC (version 0.11.5)