FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152273046
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA679350.036.3305749
GCCGTAT683800.035.92976847
CGCCGTA766750.032.48822446
CCGTATC760550.032.41952548
TCGCCGT785350.031.70160945
TCGAACG373000.031.25403640
TGGTCGC816700.030.63304542
GCGTCGT825100.030.01589611
GATCGGA844450.029.5091721
TTGAACG419400.029.0948167
TCGTGTA849700.028.72840714
GTATCAT872850.028.5223750
GTCGCCG877550.028.49317444
GTCGTGT868400.028.193313
GTAGATC901250.028.12445631
CGGTAAC418850.027.93010145
ACGGTAA422150.027.777644
CGTCGTG894700.027.71389412
GTCGAAC424000.027.63203239
AGAGCGT914300.027.2694058
Produced by FastQC (version 0.11.5)