FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76247362
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG85100.018.32594345
CGTTTCG100950.018.2136133
CGTATGC97600.015.97897446
CTCGTAT96550.015.61647144
TATGCCG99800.015.3153448
CGTATCG58300.012.9715153
ACACGTC142350.012.84299213
TCTCGTA126500.012.41008243
GTATCGA67850.012.3163114
AGTCACG148700.012.273519528
AATCTCG138000.011.97497441
GTCACGT155750.011.62902729
ACGTTTC166200.011.31210632
GATCGGA151700.011.2294131
CCCGTAT91700.010.8899091
ACGTCTG171750.010.88555615
TTTCGGA176700.010.75627835
CGGAATC171900.010.69583738
ATCTCGT150150.010.593163542
TATATCG75200.010.55010935
Produced by FastQC (version 0.11.5)