FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76247362
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA77000.015.27819550
GCGTCGT84500.014.37920712
GCCGTAT88000.013.75964548
CGTATCG62300.012.1294353
GTATCGA66100.011.7978974
CGACTAA98600.010.74186928
GCGCTAA63700.010.504683542
AATCGGG106000.010.2867725
CGACCGT41750.010.2464341
CCCGTAT90350.010.2332641
GATCGGA125500.010.0618132
TCGCCGT116350.010.0211646
CCGTATC123800.09.83692549
GACGCTA74000.09.652424
TGGTCGC124050.09.62110843
CGCTAAG74100.09.58947343
CTCGGAT187600.09.5810361
CGCCGTA125550.09.56175547
TATATCG77600.09.55665635
CGACGAT56300.09.49797251
Produced by FastQC (version 0.11.5)