FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61182972
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA882790.14428687772800575TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA878360.14356282006045734TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG243150.038.8096351
TCGTATG342800.030.92401545
CGTATGC352200.030.12867446
CTCGTAT350650.029.90707244
TATGCCG354250.029.8179948
CGTATCT351950.029.3936139
GTTCCGT363000.029.19842135
ACACGTC384250.028.63742313
TTCCGTA373000.028.39712336
GATCGGA374650.028.3130871
TCCGTAT373150.028.25226437
TCTCGTA370700.028.17782243
TATCTCG369150.028.17359541
CCGTATC377500.027.45937338
ACGTCTG403100.027.29859515
ATCTCGT387550.027.07660742
AGTTCCG406650.026.78547134
ATCGGAA407800.026.1274812
CACGTCT424250.025.8803714
CACACGT440800.025.12787812
Produced by FastQC (version 0.11.5)