FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85732771
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA104550.021.91921450
GCCGTAT110900.020.53854248
CGCCGTA114900.019.94523247
GCGTCGT141250.017.25963612
CCGTATC140750.016.58229649
GATCGGA159600.015.5450972
TCGCCGT146850.015.53488346
TGGTCGC162800.014.48710943
CGTCGTG175050.014.22851413
AGCGTCG176150.014.05930711
GTCGTGT180050.013.73596914
TCGTGTA183150.013.64380315
GTCGCCG185000.013.21847645
AGAGCGT190250.013.1892279
CGTGTAG193700.013.100916
ATCGGAA193250.012.9528773
AGATCGG194300.012.6212841
GAGCGTC219500.011.28049610
GGTCGCC222250.011.03457144
GTAGATC230600.010.91002232
Produced by FastQC (version 0.11.5)