FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156756187
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2913300.18584912377334106TruSeq Adapter, Index 4 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1817610.11595140420199172TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1178550.036.39530643
CTCGTAT1184150.035.62569842
CGTATGC1254000.034.17715544
GATCGGA1328600.034.058151
TATGCCG1262650.033.99432846
TCTCGTA1233200.033.9366941
ACACGTC1332450.033.7466813
AATCTCG1263000.033.3689739
ATCTCGT1280800.032.74833340
ATCGGAA1382650.032.557692
ACGTCTG1398550.032.09228515
CACGTCT1440000.031.2333914
CACACGT1460050.030.8413712
TCGGAAG1497300.030.1378213
ATGCCGT1433900.029.9986147
AGATCGG741900.029.5818521
GCCGTCT1456650.029.46609749
CCGTCTT1459000.029.44433450
GCACACG1543900.029.33541311
CGTCTGA1550000.029.0254716
Produced by FastQC (version 0.11.5)