FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156756187
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA229300.041.33549515
GCATACG252550.039.90202314
ATACGAG239100.038.290616
CGGCATA263700.036.72913412
AGATCGG695500.030.6556151
CGTATCA701150.030.06731850
TACGAGA286100.029.56564517
GCCGTAT792750.026.8367448
CGCCGTA816100.026.00073647
TCGCCGT809150.025.91116546
GATCGGA993650.025.6053071
TGGTCGC821600.025.53081943
ACGAGAT332950.025.17129318
CGAGATT330500.024.96177519
GCGTCGT859100.024.84286712
CCGTATC877100.024.32888449
ATCGGAA1044700.024.2949772
CGTCGTG921950.023.31818613
AGCGTCG952850.023.20859710
GTATCAT923000.023.1526951
Produced by FastQC (version 0.11.5)