FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128802955
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3206390.24893761171861312TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1052200.047.08070443
CGTATGC1064400.046.51431344
CTCGTAT1067750.046.187942
TATGCCG1078250.045.82221246
TCTCGTA1103700.044.40120741
ACACGTC1166600.043.757513
GATCGGA1196250.043.203761
ATCTCGT1144150.042.8591440
ACGTCTG1223200.041.614115
ATCGGAA1246100.041.4064982
CACGTCT1256850.040.56876414
ATGCCGT1237250.040.07190747
CACTTAG1248450.040.0289431
ACTTAGG1253400.039.93319732
CACACGT1288200.039.67914212
TTAGGCA1264750.039.4284634
GTATGCC1270850.039.0167145
CCGTCTT1273650.038.8318550
GCCGTCT1283100.038.56509449
TCGGAAG1341200.038.544223
Produced by FastQC (version 0.11.5)